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pgl3 mmp2  (Addgene inc)
90
Addgene inc pgl3 mmp2
(Ai–ii) pictomicrographs show the expression of BMI1 ( pointing to brown staining) in CaP tissues of African- American and Caucasian by the IHC analysis and box plot shows the comparative average BMI1-postive staining score in Caucasian and African-American tumor specimens (Bi) Box plots shows the mRNA expression in matched samples of normal vs. tumor in African American patients. (Bii) pictomicrographs show the expression of BMI1 in the lymph node metastatic tissues (Mets) of CaP patients shows as assessed by (IHC) analysis (Biii) Dot plots show correlation of BMI1 with metastasis of CaP in a large cohort of patients as analyzed using mining of oncomine and GEO data (Ci–ii) box plot graphs show the mRNA expression of BMI1, AR, PTEN, INK4A/p16 and metastatic genes (Cyclin D1, VEGF, c-MYC) in the metastatic tissues (Mets) of African-American and Caucasian patients as assessed by quantitative real-time PCR (qRT-PCR) analysis. (Di–ii) histograms showing the recruitment of BMI1 on the promoter of PTEN gene in African-American indolent CaP model (RCC7/T) and Caucasian CRPC (22Rν1) and brain metastasis model (DU145) as assessed by chromatin-immunoprecipitation (ChIP) analysis. (Ei–v) histograms showing the effect of BMI1-targeting (by siRNA) on the promoter activities of VEGF, <t>MMP2,</t> MMP9, BCl2 genes and transcriptional activity of NFκB transcriptional factor in bone metastasis CaP model of African-Americans as assessed by dual-luciferase reporter assays.
Pgl3 Mmp2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(Ai–ii) pictomicrographs show the expression of BMI1 ( pointing to brown staining) in CaP tissues of African- American and Caucasian by the IHC analysis and box plot shows the comparative average BMI1-postive staining score in Caucasian and African-American tumor specimens (Bi) Box plots shows the mRNA expression in matched samples of normal vs. tumor in African American patients. (Bii) pictomicrographs show the expression of BMI1 in the lymph node metastatic tissues (Mets) of CaP patients shows as assessed by (IHC) analysis (Biii) Dot plots show correlation of BMI1 with metastasis of CaP in a large cohort of patients as analyzed using mining of oncomine and GEO data (Ci–ii) box plot graphs show the mRNA expression of BMI1, AR, PTEN, INK4A/p16 and metastatic genes (Cyclin D1, VEGF, c-MYC) in the metastatic tissues (Mets) of African-American and Caucasian patients as assessed by quantitative real-time PCR (qRT-PCR) analysis. (Di–ii) histograms showing the recruitment of BMI1 on the promoter of PTEN gene in African-American indolent CaP model (RCC7/T) and Caucasian CRPC (22Rν1) and brain metastasis model (DU145) as assessed by chromatin-immunoprecipitation (ChIP) analysis. (Ei–v) histograms showing the effect of BMI1-targeting (by siRNA) on the promoter activities of VEGF, MMP2, MMP9, BCl2 genes and transcriptional activity of NFκB transcriptional factor in bone metastasis CaP model of African-Americans as assessed by dual-luciferase reporter assays.

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: BMI1 drives metastasis of prostate cancer in Caucasian and African-American men and is a potential therapeutic target: hypothesis tested in race-specific models

doi: 10.1158/1078-0432.CCR-18-1394

Figure Lengend Snippet: (Ai–ii) pictomicrographs show the expression of BMI1 ( pointing to brown staining) in CaP tissues of African- American and Caucasian by the IHC analysis and box plot shows the comparative average BMI1-postive staining score in Caucasian and African-American tumor specimens (Bi) Box plots shows the mRNA expression in matched samples of normal vs. tumor in African American patients. (Bii) pictomicrographs show the expression of BMI1 in the lymph node metastatic tissues (Mets) of CaP patients shows as assessed by (IHC) analysis (Biii) Dot plots show correlation of BMI1 with metastasis of CaP in a large cohort of patients as analyzed using mining of oncomine and GEO data (Ci–ii) box plot graphs show the mRNA expression of BMI1, AR, PTEN, INK4A/p16 and metastatic genes (Cyclin D1, VEGF, c-MYC) in the metastatic tissues (Mets) of African-American and Caucasian patients as assessed by quantitative real-time PCR (qRT-PCR) analysis. (Di–ii) histograms showing the recruitment of BMI1 on the promoter of PTEN gene in African-American indolent CaP model (RCC7/T) and Caucasian CRPC (22Rν1) and brain metastasis model (DU145) as assessed by chromatin-immunoprecipitation (ChIP) analysis. (Ei–v) histograms showing the effect of BMI1-targeting (by siRNA) on the promoter activities of VEGF, MMP2, MMP9, BCl2 genes and transcriptional activity of NFκB transcriptional factor in bone metastasis CaP model of African-Americans as assessed by dual-luciferase reporter assays.

Article Snippet: We used following plasmids pTK-TCF-Luc (Upstate Laboratories, Lake Placid, NY); pGL3-MMP2, pGL3-VEGF, pGL3-MYC (Addgene, Cambridge, MA) and pGL3-BMI1 (gifted by Goberdhan Dimri, George Washington University, Washington DC).

Techniques: Expressing, Staining, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Chromatin Immunoprecipitation, Activity Assay, Luciferase